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Fig. 3. The generation of mutations in the Cp190 gene. (A) A schematic representation of the Cp190 locus. Genes are highlighted in blue, and the position of the l(3)neo43 P-element insertion is shown in green. The extent of the Df(3R)P280NR27 is indicated by the black line. (B) A western blot showing the levels of CP190 and CP60 in wild type, and Cp1901 and Cp1902 homozygous mutant brains, and in Cp1902 homozygous mutant brains that also express either a Pubq-CP190 or Pubq-190{Delta}M transgene (as indicated above each lane). Note that both CP190 and CP60 appeared to migrate slightly abnormally in the pUbq-CP190 over-expressing lane; this was because of a defect in this gel and it was not seen when this sample was re-run on a different gel.





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