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Fig. 6. CP190 ${Delta}$ M does not interact with centrosomes or microtubules. (A) Schematic representation of the CP190 protein. The previously identified centrosomal and microtubule targeting domain is shown in red, whereas the region deleted in CP190 ${Delta}$ M is indicated by the black bars above the protein (aa309-aa541). (B) A western blot of a microtubule spin-down experiment probed with anti-CP190 and anti-CP60 antibodies. In the absence of taxol in the embryo extract, microtubules do not form. Under these conditions, when the extract is centrifuged on a sucrose cushion, CP190, CP190 ${Delta}$ M and CP60 all remain in the supernatant (S) and are not detectable in the pelleted material (P). In the presence of taxol, microtubules are polymerised in the extract. Under these conditions, both CP190 and CP60 co-sediment with microtubules through the sucrose cushion and are found in the pellet. CP190 ${Delta}$ M, however, does not co-sediment with the microtubules and is not detectable in the pellet. (C) The localisation of CP190 (black and white panel, red in merged image), microtubules (green in merged image) and DNA (blue in merged image) in Cp190 mutant brains that also contain a transgene expressing either full-length CP190 (pUbq-CP190 – top panels) or CP190 ${Delta}$ M (pUbq-CP190 ${Delta}$ M – bottom panels). Only the full-length CP190 is concentrated at centrosomes. Scale bar: 10 µm.

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