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Fig. 7. Knockdown of hFis1 in COS-7 cells leads to the formation of mitochondrial tubule extensions. COS-7 cells were transfected with vector alone (scrambled sequence; control) or vector encoding small interfering RNAs specific for hFis1 knockdown. (A) At 72 hours after transfection, mitochondria were isolated and subjected to SDS-PAGE and western blot analysis using antibodies specific for hFis1 and Tom40 (as control; left). The amount of hFis1 knockdown was quantified (right). (B) Representative mitochondrial morphologies observed in cells transfected with control and hFis1 small interfering RNAs as described in (A). Scale bar, 5 µm.
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