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Fig. 1. Effects of EGF on GJIC and the Cx43 phosphorylation pattern in IAR20 cells. GJIC was measured by quantitative scrape loading. For Cx43 band-pattern examination, cell lysates were prepared as indicated and subjected to SDS-PAGE and western blotting with anti-Cx43 antibodies. (A) Cells were exposed to 50 ng ml–1 EGF for different lengths of time. (B) Cells were exposed to increasing concentrations of EGF for 30 minutes. (C) Cells were exposed to increasing concentrations of PD98059 for 30 minutes, then to EGF at a final concentration of 50 ng ml–1 and incubated for 30 minutes, in the sustained presence of PD98059.





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