|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
|
Fig. 5. Effect of MG132 and leupeptin on EGF-induced Cx43 degradation. (A) IAR20 cells were treated with 10 µg ml–1 cycloheximide (chx) in combination with either 100 ng ml–1 EGF, 100 ng ml–1 EGF and 10 µM MG132, or 100 ng ml–1 EGF and 100 µM leupeptin. Cell lysates were prepared at the indicated time points and equal amounts of total cell protein were subjected to SDS-PAGE. Cx43 was detected by western blotting with anti-Cx43 antibodies. (B) The Cx43 band intensities on gels shown in (A) were measured using Scion Image. The Cx43 band intensities were plotted relative to the intensity of Cx43 bands in untreated cells.
|
