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Fig. 8. Cx43 is hyperphosphorylated and ubiquitinated in the presence of hypertonic sucrose medium. (A) Cells were either left untreated or treated with 50 ng ml–1 EGF for 15 minutes in the presence or absence of 0.45 M sucrose as indicated. Cell lysates were prepared and equal amounts of total cell protein were subjected to SDS-PAGE. Cx43 was detected by western blotting with anti-Cx43 antibodies. (B) Cells were either left untreated or treated with 50 ng ml–1 EGF for 15 minutes in the presence or absence of 0.45 M sucrose as indicated. Cell lysates were subjected to immunoprecipitation with anti-Cx43 antibodies. Equal amounts of total cell protein were subjected to SDS-PAGE and ubiquitin and Cx43 were detected by western blotting using affinity-purified anti-ubiquitin antibodies (top) and anti-Cx43 antibodies (bottom).
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