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Fig. 10. Schematic representation of the distribution of SCP3 and Rad21 from pachytene up to telophase I (A); their localization and that of other proteins at metaphase I centromeres (B); and their three-dimensional localization relative to sister kinetochores (C). (A) One chromosome is depicted in light grey while its homologue is depicted in darker grey. Centromere labelling is red, and SCP3/Rad21 is green. Regions of overlap are in yellow. From middle diplotene up to metaphase I there is a continuous loss of SCP3/Rad21 from chromosome arms, and an accumulation at centromeres, that in turn change their structure. During diakinesis SCP3/Rad21 also appear as accumulations on desynapsed LEs and as large agglomerates in the nucleoplasm. SCP3 and Rad21 remain at the interchromatid domain of metaphase I bivalents as small patches that are lost during the metaphase I/anaphase I transition. A population of SCP3/Rad21 persist at anaphase I centromeres but is released when sister kinetochores separate during telophase I. (B) A metaphase I centromere. Different proteins are aligned among them in side and top views. Trilaminar sister kinetochores are tightly associated at the surface of the centromere, and associated with microtubules emanating from the same pole. A single dashed line aligned with sister kinetochores has been included to facilitate the analysis of the relative distribution of these proteins. (C) Interpretation showing how sister kinetochores (red) are associated and supported by the `double-cornet'-shaped inner centromere domain containing SCP3, SCP2, Rad21, INCENP and aurora-B.
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