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Fig. 3. Selected metaphase I autosomal (A-D,I) and sex (E,H) bivalents, and sex univalents (F,G,J). SCP3 is labelled in green, centromeres in red in A-G, Rad21 in red in H-J, and DNA in blue. In the autosomal bivalents SCP3 appears highly enriched at homologous centromeres (red arrowheads in A). A fainter SCP3 labelling is also found at the interchromatid domain (red arrows in D). Note however that this labelling does not reach the distal chromosome ends (red arrows in B), and that it is interrupted at chiasma sites (white arrows in C). In the sex bivalent the SCP3 labelling at the centromere of the X chromosome (X) (red arrowhead in E) is bigger than that found at the centromere of the Y chromosome (Y). The SCP3 labelling at the interchromatid domain of the sex chromosomes is larger and more continuous (red arrows in E) than that observed in autosomal bivalents independently of whether they appear as a bivalent (E,H) or as univalents (F,G,J). In E the chiasma site in one sex bivalent is indicated by an arrow and a large SCP3 agglomerate (indicated by an arrowhead) lies in the cytoplasm near the sex bivalent. (H,I) Rad21 colocalizes with SCP3 in sex (H) and autosomal (I) bivalents, and for instance in the univalent X chromosome (J). Scale bar: 2 µm.
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