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Fig. 5. The ultrastructure of the inner centromere domain is revealed by the Os-PPD technique. (A) Section of a metaphase I spermatocyte. Condensed bivalents show very low contrast, while centromeres show high contrast. Bundles of kinetochore microtubules (MTs) are present. A semithin section of a metaphase I spermatocyte viewed by phase-contrast microscopy is shown in the inset. (B,C) Enlarged centromeres facing opposite poles where the outer (black arrowheads) and middle electron-lucent (white arrowheads) kinetochore plates are visible. Beneath kinetochores (K), a highly contrasted T-shaped region, representing the inner centromere domain (In), is observed. Scale bar: (A) 2.5 µm; (B,C) 0.5 µm.





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