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Fig. 8. SCP3 and Rad21 are lost from chromosome arms during the metaphase I/anaphase I transition, but are retained at centromeres until telophase I. SCP3 is labelled in green, centromeres in red in E, F, H, I, Rad21 in red in K, and DNA in blue. (A-C) Partial projections from different focal planes throughout an anaphase I spermatocyte. SCP3 has been lost from chromosome arms but is still highly enriched at homologous centromeres. A large SCP3 agglomerate (arrowhead in A) is found in the cytoplasm. (D-F) Top view of an anaphase I cell pole. SCP3 rings (arrowheads) are observed at some centromeres where the two sister kinetochores lye side by side. (G-I) Partial projection from two focal planes of a telophase I cell pole. SCP3 is usually found as small bars that may appear between the slightly separated sister kinetochores (white arrowheads) or displaced from them (blue arrowheads). (J-L) Single focal plane of a telophase I cell pole. Rad21 and SCP3 colocalize at bars. Scale bars: (A-C) 2.5 µm; (D-L) 5 µm.

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