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Fig. 9. SCP3 persists at chromocentres during interkinesis, but is not present at centromeres of prometaphase II or metaphase II chromosomes. SCP3 is labelled in green, centromeres in red in A,C,D,E,G,H,I, Rad21 in red in K, and DNA in blue. (A,B) Single focal plane of an interkinesis nucleus where SCP3 is found as elongated bars at chromocentres but displaced from pairs of sister kinetochores. (C,D) Single focal plane throughout a prometaphase II spermatocyte. SCP3 is not present at centromeres. Sister kinetochores are separated at the centromere of an unaligned chromosome (arrow; and see inset). (E,F) Partial projection of two focal planes throughout a metaphase II spermatocyte. SCP3 is not present at centromeres where sister kinetochores are facing opposite poles. (G,H) Selected metaphase II chromosomes. Sister kinetochores occupy opposite locations at the centromere. (I,J) Partial projection of three focal planes throughout an early anaphase II spermatocyte. No SCP3 labelling is found between segregating chromatids. (K,L) Single focal plane of a metaphase II spermatocyte. Rad21 is not present at all. Scale bars: (A-F) 5 µm; (G,H) 2 µm; (I-L) 3 µm.





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