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Fig. 12. Effect of PKA inhibition by H89 on sperm forward motility of swim-up-selected spermatozoa stimulated with LY294002 and dbcAMP. Swim-up-selected sperm samples were incubated for 1 hour in the presence of dbcAMP (1 mM), LY294002 (10 µM) and H89 (50 µM) alone or in combination, and forward and rapid motility were evaluated by CASA. Data represent means±s.e.m. of five different experiments. *P<0.01 versus respective stimulus with H89 and #P<0.01 versus respective controls (C) with or without H89; §P<0.01 versus C without H89; Student's t-test for paired data.





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