|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
|
Fig. 8. Effect of LY294002 on localization of PKA regulatory and catalytic subunits in sperm tails and heads. Western blot analysis of tail and head protein extracts from swim-up-selected spermatozoa treated or not with 10 µM LY294002, separated onto 10% SDS-PAGE and transferred to a nitrocellulose membrane. The membrane was blotted with antibodies against RIIß (A) and PKA catalytic (B) subunits. Anti-ERK2 antibody (C) was used to check for equal lane loading after stripping and re-probing of the same membrane. Jurkat cells have been used as positive controls for PKA subunits. Molecular mass markers are indicated on the right of the blots. Representative of three similar experiments.
|
