JCS00972 Supplemental Movies

Files in this Data Supplement:

• Movie 1 -

  Movie 1. (Fig. 2A) Time-lapse images of the dynamic behavior of NGFP-Cld3 in Eph4:NGFP-Cld3 cells under confluent conditions. Individual cells moved against adjacent cells while changing their shape without loosing their structural continuity. Time-lapse images were collected every 90 seconds for 90 minutes. Bars, 10 mm

• Movie 2 -

  Movie 2. (Fig. 3B) Time-lapse images of the remodeling of GFP-positive TJs in Eph4:NGFP-Cld3 cells under the confluent conditions. Owing to intercellular motility, the TJ (between arrows) decreased in length and GFP-positive granules budded from the TJ and moved into the cytoplasm (arrowheads). Time-lapse images were collected every 60 seconds for 120 minutes. Bars, 10 mm.

• Movie 3 -

  Movie 3. (Fig. 5B) Time-lapse images of the ‘eat-each-other’ endocytosis of TJs. One GFP-positive granule (arrowhead) budded off from the GFP-positive TJ, and moved into parental Eph4 cells. Time-lapse images were collected every 60 seconds for 90 minutes. Bars, 10 mm.

• Movie 4 -

  Movie 4. (Fig. 6) Time-lapse images of the process of cell detachment of adjoining CFP-claudin-1- and YFP-claudin-1-expressing MDCK cells. When these two cells were dissociated as a result of their own motility, endocytosed vesicles of TJs were double-positive for CFP and YFP. Time-lapse images were collected every 60 seconds for 20 minutes. Bars, 10 mm.

• Movie 5 -

  Movie 5. (Fig. 9B) Time-lapse images of Eph4 cells that co-express YFP-claudin-3 and ZO-1-CFP. The endocytosed TJ granules contained YFP-claudin-3 (arrows in left panel), but not ZO-1-CFP (right panel). Time-lapse images were collected every 60 seconds for 60 minutes. Bars, 15 mm.