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Fig. 11. Time-lapse observation of Eph4 cells co-expressing YFP-claudin-3 and ZO-1-CFP. In the first frame, both YFP-claudin-3 (green) and ZO-1-CFP (red) were co-concentrated at TJs. The granule endocytosed from TJs looked green (arrowheads), indicating that they did not contain ZO-1-CFP. Elapsed time is indicated at the bottom of each frame (in minutes). See Movie 5 (http://jcs.biologists.org/supplemental/). Bars, 15 µm (A); 7.5 µm (B).





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