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Fig. 9. Internalized NGFP-Cld3 and endogenous TJ proteins. (A) Eph4:NGFP-Cld3 cells were treated by immunofluorescent staining with anti-claudin-4 mAb. NGFP-Cld3 and endogenous claudin-4 were precisely co-concentrated not only at TJs but also on cytoplasmic granular structures (arrows). (B) Parental Eph4 cells were double stained with anti-claudin-3 pAb and anti-claudin-4 mAb. Many claudin-3-positive vesicles were scattered around the cytoplasm, all of which were positive for claudin-4 (arrows). (C) Parental Eph4 cells were double stained with anti-occludin mAb/anti-claudin-3 pAb (upper panels) or anti-ZO-1 mAb/anti-claudin-3 pAb (lower panels). Cytoplasmic granules carrying claudins (arrowheads) were negative for either occludin or ZO-1. Bars, 5 µm.





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