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Fig. 8. Neural derivatives of noggin-treated cells. (A) Outgrowth of cells from a sphere similar to that shown in 7B; (B,C) staining of outgrowth similar to that shown in A with antibody to 200 kDa neurofilament protein (B, phase contrast; C, indirect immunfluorescence); (D,E) staining of outgrowth similar to that shown in A with antibody to Map 2 a,b (D, phase contrast; E, indirect immunofluorescence); (F,G) cells similar to those shown in Fig. 6C following transfer to monolayer culture in the presence of serum without feeder cell support (F, phase contrast; G, indirect immunofluorescence for glial fibrillary acidic protein). Bar shown in B for A-E, 50 µM.





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