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Fig. 3. The distribution of a multi-copy S/MAR-containing transgene on somatic loops as detected by FISH. (A) The majority of green FISH signals representing non-anchoring S/MAR sequences are located on the chromatin loop and not the nuclear matrix (NM) region. There is only one FISH signal that appears to be attached to the nuclear matrix. If all inserted S/MARs anchor on the nuclear matrix, all the FISH signals should be restricted to the nuclear matrix region. (B) Diagram of (A) illustrating the position of the nuclear matrix, the chromatin loops and the FISH signals. FISH analysis clearly demonstrated that the transgenic S/MARs were present in both the loop and nuclear matrix regions demonstrating that, when multiple S/MAR copies are available in tandem fashion, they do not all associate with the nuclear matrix.
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