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Fig. 5. Distribution patterns of the S/MAR-IFNB1 insert detected by FISH. (A) Two independently integrated copies are clearly visible by FISH detection as two light spots within the nuclear matrix (indicated by the arrow). (B) Two independently integrated copies are clearly visible by FISH detection as two light spots on the loop portion (indicated by the arrow). (A,B) Even though the majority of single-copy integrations were detected on the nuclear matrix, some (a minority) were detected on the loop. (C) Approximately 20 integrated copies visualized by FISH detection as a large cluster of light blue spots located at the nuclear matrix and loop regions (indicated by the arrow). (D) Approximately 100 integrated copies visualized by FISH detection. The FISH signals of the S/MAR in the 100-copy construct were localized to both the nuclear matrix region (large light blue spot) and the loop region (multiple light spots). (C,D) Many multiple copy integrations serve as anchors (matrix location) whereas others do not (loop location). These data suggest that S/MARs are selectively used as anchors.
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