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Fig. 1. Calpastatin is decreased in the absence of Foxj1. (A) RNase protection of E15.5 lung RNA with a [32P]cRNA probe for calpastatin. Relative transcript abundance is shown in comparison with wild-type lung tissue (± s.e.m.). Samples were corrected for loading by comparison with GAPDH transcript abundance. (B) Western blot of E15.5 lung tissue with calpastatin primary antibody. Full-length tissue calpastatin is a 110 kDa protein and a truncated erythrocyte calpastatin isoform is a 70 kDa protein. GAPDH (36 kDa) abundance was used as a protein loading control. (C,D) Immunohistochemistry of foxj1+/+ (C) and foxj1-/- (D) lung tissue with primary antibody to calpastatin and peroxidase detection. Scale bar: 5 µm.





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