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Fig. 5. Ezrin is associated with the basal bodies of ciliated epithelial cells. (A) Immunoelectron microscopy of tracheal epithelium with primary antibody for ezrin demonstrates gold particles associated with basal bodies (arrows) at the cell apex. Gold particles are also associated with the apical microvilli (arrowheads). No significant gold particles are seen in association with axonemal structures (*). Scale bar: 250 nm. (B) Transmission electron microscopy of trachea from a foxj1+/+ mouse incubated with secondary gold-labeled antibody only. Microvilli (arrowheads) extend from the cell apex between axonemes. Basal bodies are localized at the cell apex (arrows). (C) Transmission electron microscopy of trachea from a foxj1-/- mouse incubated with primary antibody for ezrin and secondary gold-labeled antibody. No gold particles are detected in association with basal bodies that are distributed throughout the cytoplasm (arrows). No gold particles are associated with the microvilli that are severely flattened. No axonemal structures are present. Scale bar: 200 nm (B,C).





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