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Fig. 8. Calpain inhibition results in apical localization of ezrin and EBP-50 in foxj1^-/- trachea. (A) Immunofluorescence of untreated, E17.5 foxj1^-/- tracheal explant cultured for 96 hours with primary antibody to ezrin and acetylated ${alpha}$ -tubulin. (B) Immunofluorescence of E17.5 foxj1^-/- tracheal explant cultured for 96 hours in the presence of 10 mM calpain inhibitor II with primary antibody to ezrin and acetylated ${alpha}$ -tubulin. (C) Immunofluorescence of E17.5 foxj1^-/- tracheal explant cultured for 96 hours in the presence of 10 mM calpain inhibitor II with primary antibody to EBP-50 and acetylated ${alpha}$ -tubulin. Scale bars: 20 µm (A); 2.5 µm (B); 5 µm (C).

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