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Fig. 5. The major p120 Y phosphorylation sites are not required for E-cadherin-mediated strong cell adhesion. Hanging drop aggregation assays were conducted with p120-deficient cells expressing human p120 siRNA alone (vector), or the same cells reconstituted with murine p120 constructs expressing WT-p120 (mp120-3A) or mutant p120 (mp120-3A/7F). The latter construct contained phenylalanine (F) mutations at the seven major sites of p120 tyrosine phosphorylation. Cells were photographed on a phase-contrast light microscope (10x magnification).





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