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Fig. 11. Effects of HGF/SF on phosphorylation of ERK 1/2 and Akt proteins. Quiescent cells were treated for the indicated times with HGF/SF (20 ng/ml). One group of cells did not receive any HGF and were considered 0 hours. Immunoblot analysis was performed with the use of the (A) anti-phospho-Akt kinase antibody or (B) anti-phospho-ERK 1/2 kinase antibody. The filters used for examination of phospho-ERK 1/2 and Akt were stripped and reprobed with their respective anti-ERK 1/2 and Akt antibodies to determine equal protein loading. The bar diagrams on the right panel represent the densitometric values for the respective western blot analysis.





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