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Fig. 7. (A) Effect of PD153035, a specific and stable inhibitor of EGF receptor tyrosine phosphorylation, on EGF-induced EGFR and ERK 1/2 phosphorylation. Quiescent MDCK II cells were treated with 20 ng/ml of EGF with or without PD153035 (2.5 µM) for 30 minutes before EGF treatment. (B) Effect of PD153035 on HGF/SF-induced cell scattering in MDCKcon and MDCK5aa cells. Experiments were performed as in Fig. 6 except that one group of cells received PD 153035 (2.5 µM) 30 minutes before the addition of HGF (20 ng/ml). The top panel shows MDCKcon cells and the lower panel shows MDCK5aa cells. Original magnificatiox100.





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