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Fig. 1. Colocalisation of kAE1(R589H), kAE1(S613F) and kAE1(R901Stop) proteins with intracellular markers in non-polarised MDCKI cells. (A-C) MDCKI-kAE1(R589H), (D-F) MDCKI-kAE1(S613F) and (G-M) MDCKI-kAE1(R901Stop). Cells were treated with Bric 170 anti-AE1, followed by anti-calnexin or anti-LAMP1 antibodies and then incubated with TRITC-conjugated anti-mouse secondary antibody and FITC-conjugated anti-rabbit secondary antibody. (A,D,G,K) Visualising anti-AE1. (B,E,H) Visualising anti-calnexin. (L) Visualising anti-LAMP1. (C,F) Merged images show calnexin and kAE1(R589H) and kAE1(S613F) staining (overlaps in yellow). (I,M) Merged images show that kAE1(R901Stop) staining partially overlaps with calnexin (I) and that kAE1(R901Stop) also localises to a perinuclear region that overlaps with LAMP1 (M). Scale bar, 15 µm.





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