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Fig. 2. Surface labelling of non-polarised MDCKI-kAE1 cells with FITC-Bric 6 anti-AE1 antibody. MDCKI cells stably expressing p3.1 vector (A,B), kAE1 (C,D) or kAE1(R901Stop) (E-H) were incubated with FITC-Bric 6, which recognises an extracellular epitope on AE1. (A,C,E,G) bright field images. (B,D,F,H) fluorescence images. The majority of kAE1 cells bind FITC-Bric 6 (D), whereas the majority of kAE1(R901Stop) cells do not bind Bric 6, (F) with only a small number of obvious positive cells (H). Scale bar, 15 µm.





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