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Fig. 3. (A) Subcellular distribution of 14-3-3 ${sigma}$ -YFP and 14-3-3 ${zeta}$ -YFP in the indicated mammalian cells. (B) HaCaT and Hela cells immunostained for endogenous 14-3-3 ${sigma}$ and 14-3-3 ${zeta}$ . (C) Quantitative analysis of the subcellular distribution of 14-3-3 ${sigma}$ (red) and 14-3-3 ${zeta}$ (blue). Solid bars represent the average nuclear/cytoplasmic (F_N/F_C) ratio obtained with YFP-labelled 14-3-3 isoforms. Hatched bars represent the quantitative analysis of immunolocalisations of endogenous 14-3-3 ${sigma}$ and ${zeta}$ . Standard deviations are indicated (black lines).

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