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Fig. 4. Subcellular distribution of 14-3-3 after biochemical cell fractionation. HaCaT cells were fractionated into a cytosolic (C) and nuclear extract (N). Equal amounts of total protein from both extracts were analysed by western blotting. (A) Blot stained with specific 14-3-3{sigma} antibodies or (B) specific 14-3-3{zeta} antibodies. (C) Control probed with antibodies against known nuclear (p53), cytoplasmic (RasGAP, PKC{zeta}) and cytoskeletal (actin, {alpha}-tubulin) proteins.





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