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Fig. 5. Effect of LMB on intracellular 14-3-3 ${sigma}$ and 14-3-3 ${zeta}$ distribution. (A) HaCaT cells expressing 14-3-3 ${sigma}$ -YFP or 14-3-3 ${zeta}$ -YFP were incubated in medium with or without LMB for 5 hours. (B) Plot of the individual F_N/F_C ratios for 14-3-3 ${sigma}$ -YFP and 14-3-3 ${zeta}$ -YFP of 50 cells, with and without LMB treatment. (C) Percentage of cells with a significantly increased F_N/F_C ratio (F_N/F_C>0.45 for 14-3-3 ${sigma}$ and F_N/F_C>0.77 for 14-3-3 ${zeta}$ ). (D) HaCaT cells incubated in medium with or without LMB for 5 hours were immunostained with specific antibodies for 14-3-3 ${sigma}$ or 14-3-3 ${zeta}$ . (E) Plot of the individual F_N/F_C ratios for endogenous 14-3-3 ${sigma}$ and 14-3-3 ${zeta}$ of cells treated with and without LMB. (F) Percentage of cells with a significantly increased F_N/F_C ratio of endogenous 14-3-3 (F_N/F_C>0.31 for 14-3-3 ${sigma}$ and F_N/F_C>0.64 for 14-3-3 ${zeta}$ ).

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