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Fig. 6. FRAP analysis of nucleocytoplasmic transport of 14-3-3 proteins. (A) A region covering >75% of the nucleus (indicated by the white box) of HaCaT cells expressing 14-3-3 ${sigma}$ -YFP or 14-3-3 ${zeta}$ -YFP was photobleached, after which images were recorded at 1-second intervals. Fluorescence recovery is shown for two representative cells at the indicated times in seconds. (B) Typical normalised recovery curves with ${tau}$ _1/2 for nuclear export for 14-3-3 ${sigma}$ -YFP, 14-3-3 ${zeta}$ -YFP and YFP alone. (C) Rate constants for the nuclear import and export of 14-3-3 ${sigma}$ -YFP, 14-3-3 ${zeta}$ -YFP and YFP alone, in untreated and LMB-treated cells. At least 15 cells were measured and quantified in each experiment.

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