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Fig. 2. Cell surface labeling of CTX-FITC. CaCo-2 (A-C) and HeLa (D-I) cells were incubated with CTX-FITC (A,D,G) for 30 minutes at 4°C prior to fixation. After fixation, the cells were labeled with either GM130 (B,E) or caveolin (H), as indicated. Merged confocal images present CTX-FITC in green (C,F,I), GM130 in blue (C,F) and caveolin in red (I). Arrows identify examples of HeLa cells that express low GM1 levels. Scale bars: 16 µm (A-F) and 20 µm (G-I).





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