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Fig. 5. Quantification of CTX internalization in HeLa and CaCo-2 cells. CTX-FITC internalization to the GM130-positive Golgi (CTX-GM130 overlap; left panels) or to TfR-positive endosomes (CTX-TfR overlap; right panels) was quantified in untreated cells (black bars), and in the presence of mßCD (white bars) or genistein (grey bars) in high-GM1 HeLa cells (A), low-GM1 HeLa cells (B) and CaCo-2 cells (C), as indicated. Inhibition of CTX delivery to the Golgi with mßCD and genistein was significant (*P<0.05) in high-GM1 cells (A) but not in low GM1 (B) or CaCo-2 (C) cells.





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