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Fig. 8. mßCD, genistein-sensitive uptake of CTX to the Golgi apparatus in CaCo-2 cells supplemented with GM1. CaCo-2 cells grown in the presence of 5 mM GM1 for 4 hours followed by a 4-hour chase in regular medium were incubated with CTX-FITC for 30 minutes at 37°C (A,E,I) in regular medium (A-D) or in the presence of mßCD (E-H) or genistein (I-L). The Golgi apparatus was labeled with GM130 (B,F,J) and endosomes with TfR (C,G,K). Merged confocal images (D,H,L) present CTX-FITC in green, GM130 in red and TfR in blue with colocalization between CTX-FITC and GM130 in yellow and between CTX-FITC and TfR in cyan. Distinct colocalization of internalized CTX with Golgi elements can be seen in untreated cells (A-D). Scale bar: 8 µm.





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