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Fig. 2. Diminishing ß3 Y747 PSSA staining during adhesion. K{alpha}Vß3 WT (A-C,E-G,I,J) and K{alpha}Vß3 Y747,759F (D,H) cells were adhered to vitronectin-coated coverslips in the presence of PMA (10 ng/ml) for 10 (A,D,E,H,I) 30 (B,F) or 70 (C,G,J) minutes. Cells were stained with rhodamine phalloidin to detect actin organization (A-D) and also ß3 Y747 PSSA with FITC-conjugated secondary antibody (E-H) as described in Materials and Methods. I(1-5) and J(1-5) show replicate merged images of K{alpha}Vß3 WT cells following adhesion for 10 (I) or 70 (J) minutes followed by costaining with rhodamine phalloidin (red) and ß3 Y747 PSSA (green). Bar, 5 µm.





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