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Fig. 4. ß3 Y747 PSSA localization in murine BMMs. Wild-type murine BMMs were adhered to vitronectin-coated coverslips in the presence of PMA (10 ng/ml) (A,B) or Y27632 (10 µM) (C,D). Cells were stained with ß3 Y747 PSSA antibody and FITC-conjugated secondary antibody as described in Materials and Methods at 10- (A,C) and 30-minute (B,D) adhesion time points. Bar, 10 µm.





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