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Fig. 5. Arp3 organizes in adherent K{alpha}Vß3 WT cells. K{alpha}Vß3 Y747,759F (A-D) and K{alpha}Vß3 WT (E-L) cells were adhered to vitronectin- (A-C,E-L) or fibronectin-coated (D) coverslips in the presence of PMA alone (10 ng/ml) (A-G) or in combination with Y27632 (10 µM) (H), TAT-Yp (100 µM) (I,K) or TAT-Y/F (100 µM) (J,L) peptides for 10 (A,E), 30 (B,D,F) or 70 (C,G-L) minutes. Cells were stained with Arp3 antibody and FITC-conjugated secondary antibody (A-J) or rhodamine phalloidin (K,L) as described in Materials and Methods. Bar, 5 µm.





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