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Fig. 6. Phosphorylated Pyk2 colocalizes with ß₃ in mature adhesion contacts. K ${alpha}$ _Vß₃ WT (A-I) and K ${alpha}$ _Vß₃ Y747,759F (J-R) cells were adhered to vitronectin-coated coverslips in the presence of PMA (10 ng/ml) for 10, 30 or 70 minutes. Cells were double-labeled as described in Materials and Methods with anti-ß₃ monoclonal antibody (A-C,J-L) and Pyk2 Y402 PSSA (D-F,M-O) with TRITC-conjugated anti-mouse (red, ß₃) and FITC-conjugated anti-rabbit (green, Pyk2 Y402 PSSA) secondary antibodies. Merging of images (G-I,P-R) reveals the organization of Pyk2 Y402 PSSA into podosomes (F) and colocalization of total ß₃ and Pyk2 Y402 PSSA (I) after 70 minutes of adhesion in K ${alpha}$ _Vß₃ WT cells, but not in K ${alpha}$ _Vß₃ Y747,759F cells (O,R). Bar, 5 µm.

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