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Fig. 7. Pyk2, Arp3 and vinculin are present in {alpha}Vß3 adhesion contacts. K{alpha}Vß3 WT cells were adhered to vitronectin-coated coverslips for 70 minutes in the presence of PMA (10 ng/ml). Cells were double-labeled as described in Materials and Methods for vinculin and Arp3 (A-C), Pyk2 and Arp3 (D-F), or vinculin and Pyk2 Y402 PSSA (G-I) antibodies with TRITC-conjugated anti-mouse and FITC-conjugated anti-rabbit secondary antibodies. Merging of images (C,F,I) reveals colocalization of the respective proteins in adhesion contacts. Bar, 5 µm.





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