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Fig. 2. Dynamics of actin foci in live cells as seem by interference reflection microscopy (IRM). (A-H) The ventral membrane of a quiescent Dictyostelium cell was analyzed at various time points, as indicated. (A'-H') The distance between the cell and the substratum is indicated as tone in IRM images. While most of the ventral membrane was gray, white rings were observed surrounding actin foci (1, 2 and 3). Scale bar: 5 µm. (I) Enlarged images of the actin foci numbered 1, 2 and 3 in A-H. (J) Intensity of the tone in the area enclosed by the bars across the white ring at the actin foci numbered 1, 2 and 3. The tone at the actin foci is darker than other areas, demonstrating that the actin foci were closer to the substratum than the rest of the ventral membrane. A movie is available (Movie 1, http://jcs.biologists.org/supplemental/).
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