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Fig. 6. Traction force was transmitted to the substratum through actin foci. (A-C) Simultaneous observations of actin foci and bead movements beneath the cell. Time 0 indicates the beginning of the observation. Red areas represent the actin foci and green dots represent the position of the beads. Arrows represent the vectors of the bead movements during the previous 4.5 seconds. The length of the arrows is three times as long as actual displacement of the beads. Interestingly, extensive movement of the beads was observed only around the actin foci. A soft silicone substratum (about 4 nN/µm in stiffness) was used in order to exaggerate the movement of the beads, as described previously (Uchida et al., 2003). Scale bar: 5 µm. (D) Time-lapse images of the areas numbered 1, 2 and 3 in B and C. Around the actin foci, extensive bead movements were observed.





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