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Fig. 2. Temporal effects of BMP-7 on muscle satellite cell differentiation. Twenty-four hours after seeding, at 2x104/cm2, cultures of primary muscle satellite cells were shifted to differentiation media (day 0, d0). BMP-7 (400 ng/ml) was added at various times over the 6-day culture period as indicated by the solid line in the outline of the experimental design. At the end of 6 days, the cultures were stained for the expression of MHC (black/brown) or for ALP activity (blue). Results from a representative experiment are shown in the upper panel. Scale bar, 250 µm.





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