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Fig. 3. Stage-dependent localization of lipid bodies in P. falciparum-infected erythrocyte. The erythrocytes infected with tightly synchronized Dd2 were stained with DAPI and the lipophilic dye, Nile Red (I) and Bodipy 493/503 (II) at the stages of (A) ring, (B) early trophozoite, (C) late trophozoite, (D) early schizont, (E) segmented schizont, (F) ruptured segmented schizont and (G) free merozoite. The first image in each set represents bright-field overlaid with DAPI (blue), the second is the Nile Red or Bodipy 493/503 staining (green) and the third is an overlay of lipid body fluorescence with DAPI signals. White areas denote regions of co-localization. Scale bar, 2 µm.





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