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Fig. 5. Co-localization of lipid bodies with the PV marker protein SERA. (A) Fluorescent microscopic analysis of schizont-stage P. falciparum-infected erythrocyte stained with Sudan III and anti-SERA Ab. From left to right, panels represent bright-field overlaid with DAPI, Sudan III, SERA and overlay of Sudan III and SERA. Yellow areas denote regions of overlap. Single-stained cells (top two rows) exhibit virtually no fluorescence with the opposing filter. (B) Confocal microscopic analysis of lipid bodies and SERA co-localization in schizont (a) and segmented schizont (b). Three sections are collected through the center of the parasite by confocal microscopy. The planes on the first and third row of each set are 0.55 µm apart from the plane shown in the central row. The images, left to right, are: bright field overlaid with DAPI (blue), Sudan III (red channel), SERA protein (green channel) and merge images of red and green channels. Scale bar, 2 µm.

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