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Fig. 7. Effect of TFP on the formation of Nile Red fluorescent lipid bodies. (A) Tightly synchronized cultures of Dd2 were treated with 100 µM (a) or 500 µM (b) TFP, or 0.1% ethanol (c) for 3 hours. (B) Parasite cultures treated with 100 µM (a) or 500 µM (b) TFP, or 0.1% ethanol (c) were re-cultured for another 6 hours. Panels in each set are, from left to right, bright field overlaid with DAPI (blue), Nile Red staining patterns (green) and merged images of DAPI and Nile Red. White areas denote regions of co-localization. Scale bar, 2 µm.
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