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Fig. 1. Distribution of endogenous long MLCK during cell division. (A) Immunoblots of HeLa whole cell lysates. Lane 1, incubation with an MLCK monoclonal antibody and lane 2, incubation with a HeLa MLCK IgG domain polyclonal antibody. Molecular weight standards are indicated in kDa. (B) Conventional fluorescence micrographs (left) and deconvolved images (right) of dividing HeLa cells stained with the HeLa MLCK IgG domain antibody. Scale bar, 10 µm. The endogenous long MLCK localizes to the cell cortex (arrows).





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