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Fig. 2. Repopulation of the mammary gland. (A) Mammary epithelial cells grown in culture showing the expression of cytokeratin (red) and vimentin (green). Mammary epithelial cells averaged 90% of the total cell population transplanted into cleared fat pads. Counterstaining, Hoescht 33258 (blue). Mammary epithelial cells were injected into cleared fat pads and the recombinants were harvested at 0 (B), 30 (C), 60 (D) and 90 (E) days after cell injection. Bars, 20 µm (A); 2 mm (B-E).





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