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Fig. 6. Analysis of the presence of point mutation in the Ha-ras-1 gene using the MAMA. The mutant-specific amplification product is 74 bp whereas the normal product is 128 bp. Lanes 1, 5, 6 and 7: mammary tumors from Group 5. Tumor in lane 1 lacks Ha-ras-1 gene mutation. Lane 2: mammary tumor from Group 1. Note absence of Ha-ras-1 gene mutation. Lanes 3 and 4: mammary tumors from Group 2. Lanes 8 and 9: normal mammary tissue from intact animals taken randomly from the colony. Note: the smaller bands in lanes 1 and 2 correspond to dimers of the primer.





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