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Fig. 2. Hallmarks of vascular development in the EBs. (A) Co-localization of TAL-1/SCL and VEGFR-2 at day 6 and 8. Note that more mature vessels did not stain positive for TAL-1. (B) At day 16, sprouting angiogenesis could be readily identified morphologically in the EBs, as fine vessels ending in a brush of lamellopodia from the endothelial cell in the distal tip of the vessel (arrow). (C) Benzidine-stained erythroblasts visualized as dark clusters (arrows) inside the CD31-positive vessels. (D) While CD31 expression was seen in sprouting vessels (arrow), vWF staining appeared in larger vessels (arrowhead). Scale bars: 100 µm.





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