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Fig. 2. Cell death induced by TAJ/TROY is accompanied by PS exposure in the membrane. 40 hours after transfection, cells were analysed by flow cytometry with FITC-labeled annexin V and propidium iodide. (bottom left) Viable cells (PI-, annexin-V/FITC-). (bottom right) Early apoptotic or paraptotic cells (PI-, annexin-V/FITC+). (top right) Non-viable, late apoptotic/necrotic cells (PI+, annexin-V/FITC+). (top left) Necrotic cells. Numbers in the quadrants indicate the proportions of cells in the corresponding areas. Data from one experiment are presented. Experiments were performed in triplicate, with similar results.